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Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing

Abstract

We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.

Type Journal
ISBN 1548-7105 (Electronic) 1548-7091 (Linking)
Authors Clark, M. B.; Mercer, T. R.; Bussotti, G.; Leonardi, T.; Haynes, K. R.; Crawford, J.; Brunck, M. E.; Cao, K. A.; Thomas, G. P.; Chen, W. Y.; Taft, R. J.; Nielsen, L. K.; Enright, A. J.; Mattick, J. S.; Dinger, M. E.;
Responsible Garvan Author A/Prof Marcel Dinger
Publisher Name NATURE METHODS
Published Date 2015-01-01 00:00:00
Published Volume 12
Published Issue 4
Published Pages 339-42
Status Published in-print
URL link to publisher's version http://www.ncbi.nlm.nih.gov/pubmed/25751143
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/12786