A rapid co-culture stamping device for studying intercellular communication
Regulation of tissue development and repair depends on communication between neighbouring cells. Recent advances in cell micro-contact printing and microfluidics have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. Nonetheless, these techniques are still complicated to perform and as a result, are seldom used by biologists. We report here development of a temporarily sealed microfluidic stamping device which utilizes a novel valve design for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell interactions. We demonstrate post-stamping cell viability of >95%, the stamping of multiple adherent cell types, and the ability to control the seeded cell density. We also show viability, proliferation and migration of cultured cells, enabling analysis of co-culture boundary conditions on cell fate. We also developed an in-vitro model of endothelial and cardiac stem cell interactions, which are thought to regulate coronary repair after myocardial injury. The stamp is fabricated using microfabrication techniques, is operated with a lab pipettor and uses very low reagent volumes of 20 mul with cell injection efficiency of >70%. This easy-to-use device provides a general strategy for micro-patterning of multiple cell types and will be important for studying cell-cell interactions in a multitude of applications.
|ISBN||2045-2322 (Electronic) 2045-2322 (Linking)|
|Authors||Hassanzadeh-Barforoushi, A.; Shemesh, J.; Farbehi, N.; Asadnia, M.; Yeoh, G. H.; Harvey, R. P.; Nordon, R. E.; Warkiani, M. E.;|
|Publisher Name||SCIENTIFIC REPORTS|
|Published Date||2016-01-01 00:00:00|
|OpenAccess Link||https://publications.gimr.garvan.org.au/download.php?13855_13581/2016-Hassanzadeh-Barforoushi-Sci Rep.pdf|