Arginine methylation catalyzed by PRMT1 is required for B cell activation and differentiation
Arginine methylation catalyzed by protein arginine methyltransferases (PRMT) is a common post-translational modification in mammalian cells, regulating many important functions including cell signalling, proliferation and differentiation. Here we show the role of PRMT1 in B-cell activation and differentiation. PRMT1 expression and activity in human and mouse peripheral B cells increases in response to in vitro or in vivo activation. Deletion of the Prmt1 gene in mature B cells establishes that although the frequency and phenotype of peripheral B cell subsets seem unaffected, immune responses to T-cell-dependent and -independent antigens are substantially reduced. In vitro activation of Prmt1-deficient B cells with a variety of mitogens results in diminished proliferation, differentiation and survival, effects that are correlated with altered signal transduction from the B cell receptor. Thus PRMT1 activity in B cells is required for correct execution of multiple processes that in turn are necessary for humoral immunity.PRMT1 is an arginine methyltransferase involved in a variety of cell functions. Here the authors delete PRMT1 specifically in mature B cells to show the importance of arginine methylation for B cell proliferation, differentiation and survival, and thereby for humoral immunity.
|ISBN||2041-1723 (Electronic) 2041-1723 (Linking)|
|Authors||Infantino, S.; Light, A.; O'Donnell, K.; Bryant, V.; Avery, D. T.; Elliott, M.; Tangye, S. G.; Belz, G.; Mackay, F.; Richard, S.; Tarlinton, D.|
|Responsible Garvan Author||Prof Stuart Tangye|
|Publisher Name||Nature Communications|
|URL link to publisher's version||https://www.ncbi.nlm.nih.gov/pubmed/29026071|
|OpenAccess link to author's accepted manuscript version||https://publications.gimr.garvan.org.au/open-access/14148|