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Cytokine Expression by T Follicular Helper Cells

Abstract

T follicular helper (Tfh) cells are a specialized subset of CD4+ T cells located within temporary structures known as germinal centers (GC) formed within B cell follicles of secondary lymphoid organs. In the GC, Tfh cells facilitate the production of high-affinity antibodies through secretion of effector cytokines, such as IL-21 and IL-4, and through cell-to-cell interactions. The flow cytometric-based method described here allows the detection of intracellular cytokines within the Tfh population of secondary lymphoid organs (e.g., spleen, lymph nodes, and lymphoid nodules such as Peyer's patches), enabling the study of Tfh responses to different stimuli in the context of immunity and autoimmunity.

Type Journal
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Jandl, C.; Loetsch, C.; King, C.
Responsible Garvan Author A/Prof Cecile King
Publisher Name Methods Mol Biol
Published Date 2017-06-07
Published Volume 1623
Published Pages 95-103
Status Published in-print
DOI 10.1007/978-1-4939-7095-7_8
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/28589350