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CCR6 defines memory B cell precursors in mouse and human germinal centers revealing light zone location and predominant low antigen affinity.

Abstract

Memory B cells (MBCs) and plasma cells (PCs) constitute the two cellular outputs of germinal center (GC) responses that together facilitate long-term humoral immunity. Although expression of the transcription factor BLIMP-1 identifies cells undergoing PC differentiation, no such marker exists for cells committed to the MBC lineage. Here, we report that the chemokine receptor CCR6 uniquely marks MBC precursors in both mouse and human GCs. CCR6(+) GC B cells were highly enriched within the GC light zone (LZ), were the most quiescent of all GC B cells, exhibited a cell-surface phenotype and gene expression signature indicative of an MBC transition, and possessed the augmented response characteristics of MBCs. MBC precursors within the GC LZ predominantly possessed a low affinity for antigen but also included cells from within the high-affinity pool. These data indicate a fundamental dichotomy between the processes that drive MBC and PC differentiation during GC responses.

Type Journal
Authors Suan, D.; Krautler, N.J.; Maag, J.L.V.; Butt, D.; Bourne, K.; Hermes, J.R.; Avery, D.T.; Young, C.; Statham, A.; Elliott, M.; Dinger, M.E.; Basten, A.; Tangye, S.G.; Brink, R.
Responsible Garvan Author Prof Robert Brink
Publisher Name IMMUNITY
Published Date 2017-12-19 00:00:00
Published Volume 47
Published Issue 6
Published Pages 1142-1153
Status Published in-print