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Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver


Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance.

Type Journal
ISBN 1932-6203 (Electronic) 1932-6203 (Linking)
Authors Nagarajan, S. R.; Brandon, A. E.; McKenna, J. A.; Shtein, H. C.; Nguyen, T. Q.; Suryana, E.; Poronnik, P.; Cooney, G. J.; Saunders, D. N.; Hoy, A. J.
Publisher Name PLoS One
Published Date 2017-03-22
Published Volume 12
Published Issue 3
Published Pages e0174431
Status Published in-print
DOI 10.1371/journal.pone.0174431
URL link to publisher's version
OpenAccess link to author's accepted manuscript version