Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.
|ISBN||2041-1723 (Electronic) 2041-1723 (Linking)|
|Authors||Lagendijk, A. K.; Gomez, G. A.; Baek, S.; Hesselson, D.; Hughes, W. E.; Paterson, S.; Conway, D. E.; Belting, H. G.; Affolter, M.; Smith, K. A.; Schwartz, M. A.; Yap, A. S.; Hogan, B. M.|
|Responsible Garvan Author|
|Publisher Name||Nature Communications|
|URL link to publisher's version||https://www.ncbi.nlm.nih.gov/pubmed/29123087|
|OpenAccess link to author's accepted manuscript version||https://publications.gimr.garvan.org.au/open-access/14357|