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Structural basis of antigen recognition: crystal structure of duck egg lysozyme

Abstract

Duck egg lysozyme (DEL) is a widely used model antigen owing to its capacity to bind with differential affinity to anti-chicken egg lysozyme antibodies. However, no structures of DEL have so far been reported, and the situation had been complicated by the presence of multiple isoforms and conflicting reports of primary sequence. Here, the structures of two DEL isoforms from the eggs of the commonly used Pekin duck (Anas platyrhynchos) are reported. Using structural analyses in combination with mass spectrometry, non-ambiguous DEL primary sequences are reported. Furthermore, the structures and sequences determined here enable rationalization of the binding affinity of DEL for well documented landmark anti-lysozyme antibodies.

Type Journal
ISBN 2059-7983 (Electronic) 2059-7983 (Linking)
Authors Langley, D. B.; Crossett, B.; Schofield, P.; Jackson, J.; Zeraati, M.; Maltby, D.; Christie, M.; Burnett, D.; Brink, R.; Goodnow, C.; Christ, D.
Responsible Garvan Author A/Prof Daniel Christ
Publisher Name Acta Crystallographica Section D-Structural Biology
Published Date 2017-11-01
Published Volume 73
Published Issue Pt 11
Published Pages 910-920
Status Published in-print
DOI 10.1107/S2059798317013730
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/29095163