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The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio)

Abstract

Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.

Type Journal
ISBN 2167-8359 (Print)
Authors Usui, T.; Noble, D. W. A.; O'Dea, R. E.; Fangmeier, M. L.; Lagisz, M.; Hesselson, D.; Nakagawa, S.
Responsible Garvan Author Dr Daniel Hesselson
Publisher Name PeerJ
Published Date 2018-01-17 00:00:00
Published Volume 6
Published Pages e4292
Status Published in-print
DOI 10.7717/peerj.4292
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/29372124