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Radioimmunoassay of secretin in plasma

Abstract

A sensitive, specific and reproducible radioimmunoassay for secretin is described. Antibodies were readily produced against low microgram quantities of synthetic secretin. The secretin antibodies did not cross-react with the structurally similar G.I.P., V.I.P., or glucagon. Synthetic secretin was iodinated using Chloramine ""T"" and purified by a two-state procedure incorporating gel filtration and radient elution from a cation exchange column. Plasma samples were found to produce variable interference in the assay necessitating the incorporation of secretin-free ""blands"" for each patient's plasma. Production of secretin-free plasma was by incubation of plasma samples at 37 degrees C for 96 hours. The sensitivity of the assay was 12.5-25 pg/ml. Normal fasting secretin levels were 21 +/- S.E. 7 pg/ml. A mean rise in plasma secretin to 220 pg/ml was observed after intraduodenal acidification.

Type Journal
ISBN 0018-5043 (Print)
Authors Byrnes, D. J.;Marjason, J. P. :
Publisher Name HORMONE AND METABOLIC RESEARCH
Published Date 1976-01-01
Published Volume 8
Published Issue 5
Published Pages 361-5
Status Published in-print
URL link to publisher's version http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1086282
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/145