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GSK-3beta regulates the synaptic expression of NMDA receptors via phosphorylation of phosphatidylinositol 4 kinase type IIalpha


Deregulation of GSK-3beta is strongly implicated in a variety of serious brain conditions, such as Alzheimer disease, bipolar disorder and schizophrenia. To understand how GSK-3beta becomes dysregulated in these conditions, it is important to understand its physiological functions in the central nervous system. In this context, GSK-3beta plays a role in the induction of NMDA receptor-dependent long-term depression (LTD) and several substrates for GSK-3beta have been identified in this form of synaptic plasticity, including KLC-2, PSD-95 and tau. Stabilization of NMDA receptors at synapses has also been shown to involve GSK-3beta, but the substrates involved are currently unknown. Recent work has identified phosphatidylinositol 4 kinase type IIalpha (PI4KIIalpha) as a neuronal GSK-3beta substrate that can potentially regulate the surface expression of AMPA receptors. In the present study, we investigated the synaptic role of PI4KIIalpha in organotypic rat hippocampal slices. We found that knockdown of PI4KIIalpha has no effect on synaptic AMPA receptor-mediated synaptic transmission but substantially reduces NMDA receptor-mediated synaptic transmission. Furthermore, the ability of the selective GSK-3 inhibitor, CT99021, to reduce the amplitude of NMDA receptor-mediated currents was occluded in shRNA-PI4KIIalpha transfected neurons. The effects of knocking down PI4KIIalpha were fully rescued by a shRNA-resistant wild-type construct, but not by a mutant construct that cannot be phosphorylated by GSK-3beta. These data suggest that GSK-3beta phosphorylates PI4KIIalpha to stabilize NMDA receptors at the synapse.

Type Journal
ISBN 1460-9568 (Electronic) 0953-816X (Linking)
Authors Amici, M.; Lee, Y.; Pope, R. J. P.; Bradley, C. A.; Cole, A.; Collingridge, G. L.
Published Date 2021-10-31
Published Volume 54
Published Issue 8
Published Pages 6815-6825
Status Published in-print
DOI 10.1111/ejn.14841
URL link to publisher's version