Human transitional and IgM(low) mature naive B cells preserve permissive B-cell receptors
The level of immunoglobulin M (IgM) displayed on the surface of peripheral blood B cells exhibits a broad dynamic range and has been associated with both development and selection. To determine whether IgM surface expression associates with distinct immunoglobulin heavy-chain (IGH) repertoire properties, we performed deep IgM sequencing of peripheral blood transitional and mature naive B cells in the upper and lower quartiles of surface IgM expression for 12 healthy donors. Mature naive B cells within the lowest quartile for surface IgM expression displayed more diverse IGH features including increased complementarity-determining region 3 length, IGHJ6 segment usage and aromatic amino acids compared with mature naive B cells with high surface IgM. There were no differences between IGH repertoires for transitional B cells with high or low surface IgM. These findings suggest that a selection checkpoint during progression of transitional to mature naive B cells reduces the breadth of the IGH repertoire among high surface IgM B cells but that diversity is preserved in B cells expressing low levels of surface IgM.
|Authors||Zhang, Z., Jara, C. J., Singh, M., Xu, H., Goodnow, C. C., Jackson, K. J.,Reed, J. H.|
|Responsible Garvan Author|
|Publisher Name||IMMUNOLOGY AND CELL BIOLOGY|