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Human transitional and IgM(low) mature naive B cells preserve permissive B-cell receptors


The level of immunoglobulin M (IgM) displayed on the surface of peripheral blood B cells exhibits a broad dynamic range and has been associated with both development and selection. To determine whether IgM surface expression associates with distinct immunoglobulin heavy-chain (IGH) repertoire properties, we performed deep IgM sequencing of peripheral blood transitional and mature naive B cells in the upper and lower quartiles of surface IgM expression for 12 healthy donors. Mature naive B cells within the lowest quartile for surface IgM expression displayed more diverse IGH features including increased complementarity-determining region 3 length, IGHJ6 segment usage and aromatic amino acids compared with mature naive B cells with high surface IgM. There were no differences between IGH repertoires for transitional B cells with high or low surface IgM. These findings suggest that a selection checkpoint during progression of transitional to mature naive B cells reduces the breadth of the IGH repertoire among high surface IgM B cells but that diversity is preserved in B cells expressing low levels of surface IgM.

Type Journal
Authors Zhang, Z., Jara, C. J., Singh, M., Xu, H., Goodnow, C. C., Jackson, K. J.,Reed, J. H.
Responsible Garvan Author Dr Joanne Reed
Published Date 2021-09-30
Status Published in-print
DOI 10.1111/imcb.12478