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A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells


Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.

Type Journal
ISBN 1096-0007 (Electronic) 0014-4835 (Linking)
Authors Dewi, C. U.; Mason, M.; Cohen-Hyams, T.; Killingsworth, M. C.; Harman, D. G.; Gnanasambandapillai, V.; Liyanage, L.; O'Connor, M. D.
Responsible Garvan Author (missing name)
Published Date 2021-01-31
Published Volume 202
Published Pages 108317
Status Published in-print
DOI 10.1016/j.exer.2020.108317
URL link to publisher's version