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Identification of RNA-Binding Proteins Associated to RNA Structural Elements


RNA motifs guide the interaction with specific proteins leading to the assembly of ribonucleoprotein complexes that perform key functions in cellular processes. Internal ribosome entry site (IRES) elements are organized in structural domains that determine internal initiation of translation. In this chapter we describe a pull-down assay using streptavidin-aptamer tagged RNAs that combines RNA structure-dependent protein isolation with proteomic analysis to identify novel interactors recognizing RNA structural domains. This approach takes advantage of tRNA-scaffold guided expression, allowing the identification of factors belonging to networks involved in RNA and protein metabolism.

Type Book
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Fernandez-Chamorro, J.; Francisco-Velilla, R.; Embarc-Buh, A.; Martinez-Salas, E.
Publisher Name Methods in Molecular Biology
Published Date 2021-06-30
Published Volume 2323
Published Pages 109-119
Status Published in-print
DOI 10.1007/978-1-0716-1499-0_9
URL link to publisher's version