Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination


How temperature determines sex remains unknown. A recent hypothesis proposes that conserved cellular mechanisms (calcium and redox; 'CaRe' status) sense temperature and identify genes and regulatory pathways likely to be involved in driving sexual development. We take advantage of the unique sex determining system of the model organism, Pogona vitticeps, to assess predictions of this hypothesis. P. vitticeps has ZZ male: ZW female sex chromosomes whose influence can be overridden in genetic males by high temperatures, causing male-to-female sex reversal. We compare a developmental transcriptome series of ZWf females and temperature sex reversed ZZf females. We demonstrate that early developmental cascades differ dramatically between genetically driven and thermally driven females, later converging to produce a common outcome (ovaries). We show that genes proposed as regulators of thermosensitive sex determination play a role in temperature sex reversal. Our study greatly advances the search for the mechanisms by which temperature determines sex.

Type Journal
ISBN 1553-7404 (Electronic) 1553-7390 (Linking)
Authors Whiteley, S. L.; Holleley, C. E.; Wagner, S.; Blackburn, J.; Deveson, I. W.; Marshall Graves, J. A.; Georges, A.
Publisher Name PLOS GENET
Published Date 2021-04-30
Published Volume 17
Published Issue 4
Published Pages e1009465
Status Published in-print
DOI 10.1371/journal.pgen.1009465
URL link to publisher's version