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Early expansion of CD38+ICOS+ GC Tfh in draining lymph nodes during influenza vaccination immune response


T follicular helper (Tfh) cells provide critical help to B cells during the germinal center (GC) reaction to facilitate generation of protective humoral immunity. Accessing the human lymph node (LN) to study the commitment of CD4 T cells to GC Tfh cell differentiation during in vivo vaccine responses is difficult. We used ultrasound guided fine needle biopsy to monitor recall responses in axillary LNs to seasonal influenza vaccination in healthy volunteers. Specific expansion of GC cell subsets occurred exclusively within draining LNs five days postvaccination. Draining LN GC Tfh and precursor-Tfh cells express higher levels of CD38, ICOS, and Ki67, indicating they were significantly more activated, motile, and proliferating, compared to contralateral LN cells. These observations provide insight into the early expansion phase of the human Tfh lineage within LNs during a vaccine induced memory response and highlights early LN immune responses may not be reflected in the periphery.

Type Journal
ISBN 2589-0042 (Electronic) 2589-0042 (Linking)
Authors Law, H.; Mach, M.; Howe, A.; Obeid, S.; Milner, B.; Carey, C.; Elfis, M.; Fsadni, B.; Ognenovska, K.; Phan, T. G.; Carey, D.; Xu, Y.; Venturi, V.; Zaunders, J.; Kelleher, A. D.; Munier, C. M. L.
Publisher Name iScience
Published Date 2022-01-21
Published Volume 25
Published Issue 1
Published Pages 103656
Status Published in-print
DOI 10.1016/j.isci.2021.103656
URL link to publisher's version