Target-Specific Profiling of RNA m(5)C Methylation Level Using Amplicon Sequencing
Mapping the position and quantifying the level of 5-methylcytosine (m(5)C) as a modification in different types of cellular RNA is an important objective in the field of epitranscriptomics. Bisulfite conversion has long been the gold standard for the detection of m(5)C in DNA, but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification, and detection of candidate sites by sequencing on the Illumina MiSeq platform.
|ISBN||1940-6029 (Electronic) 1064-3745 (Linking)|
|Authors||Sibbritt, T.; Schumann, U.; Shafik, A.; Guarnacci, M.; Clark, S. J.; Preiss, T.|
|Publisher Name||Methods in Molecular Biology|
|URL link to publisher's version||https://www.ncbi.nlm.nih.gov/pubmed/34694621|