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The contribution of proline 250 (P-2') to pore diameter and ion selectivity in the human glycine receptor channel

Abstract

The glycine receptor-channel (GlyR) mediates neuronal inhibition by selectively allowing the passage of Cl(-) ions through its channel. The pore region for ion selectivity is localised to the constricted internal end of the M2 transmembrane domain. This paper investigates the contribution of the P-2' residue in determining pore diameter and ion charge selectivity of the GlyR. The deletion of this proline has been shown to decrease the anion/cation permeability ratio, with P(Cl)/P(Na) decreasing from approximately 27 to approximately 4. We show that the P-2' deletion by itself produces a GlyR with a larger pore diameter ( approximately 0.69 nm) than the wild type value ( approximately 0.54 nm). This confirms that the P-2' residue reduces pore size, which suggests that, in addition to electrostatic effects, pore size also contributes to ion-charge selectivity.

Type Journal
ISBN 0304-3940 (Print)
Authors Lee, D. J.;Keramidas, A.;Moorhouse, A. J.;Schofield, P. R.;Barry, P. H. :
Publisher Name NEUROSCIENCE LETTERS
Published Date 2003-01-01
Published Volume 351
Published Issue 3
Published Pages 196-200
Status Published in-print
URL link to publisher's version http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14623139
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/1692