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Direct stimulation of T cells by membrane vesicles from antigen-presenting cells


Activation of naive T cells generally requires T cell receptor-mediated contact with MHC-bound peptides on viable antigen-presenting cells such as dendritic cells (DC). Here evidence is presented that dissociated cell membrane fragments from a DC line can be used as an effective substitute for viable DC. Ultracentrifuged material derived from sonicates of IFN-gamma-matured DC is enriched in small membrane vesicles that closely resemble exosomes. When complexed with MHC class I-restricted specific peptide, vesicles from DC sonicates generate strong responses by purified naive CD8(+) cells in vitro in the absence of normal antigen-presenting cells and can also efficiently prime T cells for tumor rejection in vivo. Both in terms of total yields from DC and relative immunogenicity, membrane vesicles from DC sonicates are much more effective than classic exosomes and may be a valuable tool for tumor immunotherapy.

Type Journal
ISBN 0027-8424 (Print)
Authors Kovar, M.;Boyman, O.;Shen, X.;Hwang, I.;Kohler, R.;Sprent, J. :
Garvan Authors Prof Jonathan Sprent
Publisher Name PNAS
Published Date 2006-01-01 00:00:00
Published Volume 103
Published Issue 31
Published Pages 11671-6
Status Published In-print
OpenAccess Link kovar PNAS Tcells.pdf