Repertoires of aggregation-resistant human antibody domains
We recently described a method for the generation of a large human domain antibody repertoire involving combinatorial assembly of CDR building blocks from a smaller repertoire comprising a high frequency of aggregation-resistant antibody domains. Here we show that the frequency of aggregation-resistant domains in the combinatorial repertoire remained high. Furthermore, one of the antigen-binding domains selected from the combinatorial repertoire retained its binding properties through 25 cycles of thermal denaturation, suggesting that antibody domains can be created that rival the heat-resistance of thermophilic proteins such as Taq polymerase.
|Authors||Christ, D.;Famm, K.;Winter, G. :|
|Publisher Name||PROTEIN ENG DES SEL|
|Published Date||2007-01-01 00:00:00|
|OpenAccess Link||https://publications.gimr.garvan.org.au/download.php?2184_10921/07 Christ PEDS.pdf|