Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

The helix-loop-helix protein Id1 requires cyclin D1 to promote the proliferation of mammary epithelial cell acini


Overexpression of the helix-loop-helix (HLH) protein Id1 has been associated with metastasis in breast cancer, but its role in models of early breast tumorigenesis is not well characterized. We show that the down-regulation of endogenous Id1 via proteosomal degradation and relocalization from the nucleus to the cytoplasm is an early event in the formation of mammary epithelial acini. Overexpression of Id1 in both human MCF-10A and primary mouse mammary epithelial cells disrupted normal acinar development by increasing acinar volume. This occurred in an HLH domain-dependent fashion via an increase in S phase. Id1 overexpression also increased apoptosis leading to accelerated luminal clearance, and this was reversed by coexpression of the proto-oncogene Bcl2, leading to large, disorganized structures with filled lumina. Id1 overexpression was unable to increase the volume of cyclin D1(-/-) acini, indicating that Id1 is dependent on cyclin D1 for its proliferative effects. In summary, Id1 may contribute to early breast cancer by promoting excessive proliferation through cyclin D1.

Type Journal
ISBN 1538-7445 (Electronic)
Authors Caldon, C. E.;Swarbrick, A.;Lee, C. S.;Sutherland, R. L.;Musgrove, E. A. :
Responsible Garvan Author (missing name)
Published Date 2008-01-01
Published Volume 68
Published Issue 8
Published Pages 3026-36
Status Published in-print
URL link to publisher's version
OpenAccess link to author's accepted manuscript version