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The amino acid sequence of Emu osteocalcin: gas phase sequencing of Gla-containing proteins


Osteocalcin (OC), the major gamma carboxyglutamic acid (Gla)-containing protein of vertebrate bone, has been isolated from bones of the emu (Dromaius novaehollandae) and the primary structure determined by a combination of gas phase N-terminal sequencing of the intact molecule and a proteolytic fragment, and carboxypeptidase Y C-terminal sequencing. Gla residues were located by counting tritium radioactivity in fractions from the N-terminal sequencing of the tritiated/thermally decarboxylated molecule. Emu OC consists of 48 amino acid residues containing 3 Gla residues, and a single disulphide bond. The C-terminal 29 residues are identical to those of the human and sheep OC sequences. Alignment of the N-terminal sequence against those of other OCs reveals greater sequence homology with chicken OC than with mammalian OCs.

Type Journal
ISBN 0158-5231 (Print)
Authors Huq, N. L.;Tseng, A.;Chapman, G. E. :
Publisher Name Biochem Int
Published Date 1987-01-01
Published Volume 15
Published Issue 2
Published Pages 271-7
Status Published in-print
URL link to publisher's version