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Cloning and expression of full-length cDNA encoding human vitamin D receptor


Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Type Journal
ISBN 0027-8424 (Print)
Authors Baker, A. R.;McDonnell, D. P.;Hughes, M.;Crisp, T. M.;Mangelsdorf, D. J.;Haussler, M. R.;Pike, J. W.;Shine, J.;O'Malley, B. W. :
Garvan Authors Prof John Shine
Publisher Name PNAS
Published Date 1988-01-01 00:00:00
Published Volume 85
Published Issue 10
Published Pages 3294-8
Status Published in-print