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The mouse relaxin gene: nucleotide sequence and expression

Abstract

Relaxin is a polypeptide hormone that has a variety of physiological effects both on remodelling of collagen and on uterine contractility. These are most apparent during pregnancy. The sequences of relaxin cDNAs derived from ovaries of late-pregnant random-bred Swiss mice have been established. Multiple subclones obtained from three independent polymerase chain reaction experiments were found to encode relaxins which were identical except at position 11 in the A chain (Ile or Val). All mouse relaxin cDNAs expressed in the ovary during pregnancy had an extra tyrosine inserted prior to the final A chain cysteine residue, a result confirmed by direct sequencing of relaxin peptides. Whilst this tyrosine insertion must have local effects on the folding of the A chain, structure-activity studies will clarify whether it perturbs functional interaction with the relaxin receptor. We have shown that there is a single relaxin gene in the mouse genome, and that expression during pregnancy occurs in the ovary but is not detectable in the placenta, uterus or fetus.

Type Journal
ISBN 0952-5041 (Print)
Authors Evans, B. A.;John, M.;Fowler, K. J.;Summers, R. J.;Cronk, M.;Shine, J.;Tregear, G. W. :
Responsible Garvan Author Prof John Shine
Publisher Name JOURNAL OF MOLECULAR ENDOCRINOLOGY
Published Date 1993-01-01
Published Volume 10
Published Issue 1
Published Pages 15-23
Status Published in-print
URL link to publisher's version http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8452637
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/768