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Isolation and chromosomal localization of a novel human G-protein-coupled receptor (GPR3) expressed predominantly in the central nervous system


Degenerate oligonucleotide primers designed against known G-protein-coupled receptors were used in polymerase chain reaction amplification to isolate a novel receptor sequence (R4) from a rat insulinoma cell line and its human homolog (GPR3) from a human neuroblastoma cDNA library. The novel human receptor sequence is expressed in low abundance predominantly in the central nervous system and at low levels in the lung and kidney. The gene encoding GPR3 is intronless within the coding region, contains at least one intron in the 5'-untranslated region, and has been localized to chromosome 1p34.3. The activating ligand for the homologous receptors R4 and GPR3 is not known, but sequence similarity with the closely related orphan rat receptor R334 [FEBS Lett. 292:243 (1991)] suggests that R334 and the homologous receptors R4 and GPR3 probably represent two discrete molecular subtypes that interact with the same or closely related ligands.

Type Journal
ISBN 0888-7543 (Print)
Authors Iismaa, T. P.;Kiefer, J.;Liu, M. L.;Baker, E.;Sutherland, G. R.;Shine, J. :
Responsible Garvan Author Prof John Shine
Publisher Name GENOMICS
Published Date 1994-01-01
Published Volume 24
Published Issue 2
Published Pages 391-4
Status Published in-print
URL link to publisher's version