The Tissue Culture Facility is a centralised unit located at The Kinghorn Cancer Centre (TKCC) that provides a secure, clean and fully equipped area in which to perform experiments requiring eukaryotic tissue culture.
The TCF stocks all reagents and consumables required to complete eukaryotic tissue culture including serum.
17 class II biohazard hoods, including Quarantine and Primary culture
25 CO2 incubators, allocated by need and maintained by the users
Walk-in cool-room with individual storage drawers
Freezer storage at -20°C and -80°C
All temperature sensitive major equipment, with the exception of the biohazard hoods, is electronically monitored by the Garvan engineering department:
37°C warming ovens
variable temperature water-baths
low speed centrifuges, microfuges and minifuges
in house vacuum
brightfield and fluorescent microscopes, some with cameras
Countess3 cell counter, haemocytometers.
Consumables provided in the tissue culture suite
Basic culture media including DMEM, RPMI, DMEM/F12, Waymouths, 199
Tissue culture flasks, plates and dishes in a range of sizes
Sterile tubes, pipettes and plugged tips
Syringes, needles and filters
Vacuum filter units
Miscellaneous consumables e.g. parafilm, scalpels, cell scrapers
PPE – gowns/lab coats and gloves
Batch tested serum