Manu Singh

Senior Research Officer - Immunogenomics Lab

Manu Singh

I am an early career researcher in the Immunogenomics Laboratory at the Garvan Institute of Medical Research and lead a research program that develops and applies single-cell multi-omic technologies to profile the adaptive immune system. Throughout my career, I have pioneered the development and imp

Research Level

Senior Research Officer

Biography

I am an early career researcher in the Immunogenomics Laboratory at the Garvan Institute of Medical Research and lead a research program that develops and applies single-cell multi-omic technologies to profile the adaptive immune system. Throughout my career, I have pioneered the development and implementation of key technologies to profile the adaptive immune response, gaining broad expertise in immunology, cellular genomics and biotechnology development. These transformative single cell technologies have been applied to address outstanding questions in immunology, such as the clonal evolution of self-reactive B cells in human autoimmune patients.

I am an early career researcher in the Immunogenomics Laboratory at the Garvan Institute of Medical Research and lead a research program that develops and applies single-cell multi-omic technologies to profile the adaptive immune system. Throughout my career, I have pioneered the development and implementation of key technologies to profile the adaptive immune response, gaining broad expertise in immunology, cellular genomics and biotechnology development. These transformative single cell technologies have been applied to address outstanding questions in immunology, such as the clonal evolution of self-reactive B cells in human autoimmune patients.

Awards and Honours

2022-2026 NHMRC Investigator Grant EL1
2020 Oxford Nanopore travel grant
2019 Palmer Innovation prize
2019 IUIS travel grant to attend the International Congress of Immunology, Beijing, China
2018 Best early career researcher oral presentation. Oz Single Cells conference, Melbourne.
2015 JDRF international travel grant

Education

2017. PhD. Australian National University, Australia.
2010. BSc (Hons). University of Otago, New Zealand.

Selected Publications

Zhang, Z., Jara, J. C., Singh, M., Xu, H., Goodnow, C. C., Jackson, J. I. K., Reed, H. J. Human transitional and IgMlow mature naïve B cells preserve permissive B-cell receptors. Immunology and Cell Biology (2021). doi: 10.1111/imcb.12478.

   

Gottlieb, D. J., Clancy, L. E., Withers, B., McGuire, H. M., Luciani, F., Singh, M., Hughes, B., Gloss, B.,

Kliman, D., Ma, C. K. K., Panicker, S., Bishop, D., Dubosq, M. C., Li, Z., Avdic, S., Micklethwaite, K., Blyth,

  1. Prophylactic antigen-specific T-cells targeting seven viral and fungal pathogens after allogeneic

haemopoietic stem cell transplant. Clinical and Translational Immunology (2021) 10 3 e1249

 

Singh, M., Jackson, K. J. L., Wang, J. J., Schofield, P., Field, M. A., Koppstein, D., Peters, T. J., Burnett, D.

L., Rizzetto, S., Nevoltris, D., Masle-Farquhar, E., Faulks, M. L., Russell, A., Gokal, D., Hanioka, A.,

Horikawa, K., Colella, A. D., Chataway, T. K., Blackburn, J., Mercer, T. R., Langley, D. B., Goodall, D. M.,

Jefferis, R., Gangadharan Komala, M., Kelleher, A. D., Suan, D., Rischmueller, M., Christ, D., Brink, R.,

Luciani, F., Gordon, T. P., Goodnow, C. C., Reed, J. H. Lymphoma Driver Mutations in the Pathogenic

Evolution of an Iconic Human Autoantibody Cell (2020) 180 5 878-894 e19

 

Castellano-Gonzalez, G., McGuire, H. M., Luciani, F., Clancy, L. E., Li, Z., Avdic, S., Hughes, B., Singh,

M., Fazekas de St Groth, B., Renga, G., Pariano, M., Bellet, M. M., Romani, L., Gottlieb, D. J. Rapidly

expanded partially HLA DRB1-matched fungus-specific T cells mediate in vitro and in vivo antifungal

activity Blood Adv (2020) 4 14 3443-3456

 

Lee, K. H., Gowrishankar, K., Street, J., McGuire, H. M., Luciani, F., Hughes, B., Singh, M., Clancy, L. E.,

Gottlieb, D. J., Micklethwaite, K. P., Blyth, E. Ex vivo enrichment of PRAME antigen-specific T cells for

adoptive immunotherapy using CD137 activation marker selection Clinical & Translational Immunology

(2020) 9 10

 

Singh, M., Al-Eryani, G., Carswell, S., Ferguson, J.M., Blackburn, J., Barton, K., Roden, D., Luciani,

F., Phan, Giang T., Junankar, S., Jackson, K., Goodnow, C.C., Smith, M.A., Swarbrick, A. Highthroughput

targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of

lymphocytes Nat Commun (2019) 10 1 3120

 

Rizzetto, S., Koppstein, D.N.P., Samir, J., Singh, M., Reed, J.H., Cai, C.H., Lloyd, A.R., Eltahla, A.A.,

Goodnow, C.C., Luciani, F. B-cell receptor reconstruction from single-cell RNA-seq with VDJPuzzle

Bioinformatics (2018) 34 16 2846-2847

 

Wirasinha, R.C., Singh, M., Archer, S.K., Chan, A., Harrison, P.F., Goodnow, C.C., Daley, S.R.

alphabeta T-cell receptors with a central CDR3 cysteine are enriched in CD8alphaalpha intraepithelial

lymphocytes and their thymic precursors Immunol Cell Biol (2018) 96 6 553-561

 

Fisher, C.R., Sutton, H.J., Kaczmarski, J.A., McNamara, H.A., Clifton, B., Mitchell, J., Cai, Y., Dups,

J.N., D'Arcy, N.J., Singh, M., Chuah, A., Peat, T.S., Jackson, C.J., Cockburn, I.A. T-dependent B cell

responses to Plasmodium induce antibodies that form a high-avidity multivalent complex with the

circumsporozoite protein PLoS Pathog (2017) 13 7 e1006469

 

Andrews, T.D., Whittle, B., Field, M.A., Balakishnan, B., Zhang, Y., Shao, Y., Cho, V., Kirk, M., Singh,

M., Xia, Y., Hager, J., Winslade, S., Sjollema, G., Beutler, B., Enders, A., Goodnow, C.C. Massively

parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate

source for thousands of new mouse models Open Biol (2012) 2 5 120061