Manu Singh
Research Level
Biography
I am an early career researcher in the Immunogenomics Laboratory at the Garvan Institute of Medical Research and lead a research program that develops and applies single-cell multi-omic technologies to profile the adaptive immune system. Throughout my career, I have pioneered the development and implementation of key technologies to profile the adaptive immune response, gaining broad expertise in immunology, cellular genomics and biotechnology development. These transformative single cell technologies have been applied to address outstanding questions in immunology, such as the clonal evolution of self-reactive B cells in human autoimmune patients.
I am an early career researcher in the Immunogenomics Laboratory at the Garvan Institute of Medical Research and lead a research program that develops and applies single-cell multi-omic technologies to profile the adaptive immune system. Throughout my career, I have pioneered the development and implementation of key technologies to profile the adaptive immune response, gaining broad expertise in immunology, cellular genomics and biotechnology development. These transformative single cell technologies have been applied to address outstanding questions in immunology, such as the clonal evolution of self-reactive B cells in human autoimmune patients.
Awards and Honours
2020 Oxford Nanopore travel grant
2019 Palmer Innovation prize
2019 IUIS travel grant to attend the International Congress of Immunology, Beijing, China
2018 Best early career researcher oral presentation. Oz Single Cells conference, Melbourne.
2015 JDRF international travel grant
Education
2010. BSc (Hons). University of Otago, New Zealand.
Selected Publications
Zhang, Z., Jara, J. C., Singh, M., Xu, H., Goodnow, C. C., Jackson, J. I. K., Reed, H. J. Human transitional and IgMlow mature naïve B cells preserve permissive B-cell receptors. Immunology and Cell Biology (2021). doi: 10.1111/imcb.12478.
Gottlieb, D. J., Clancy, L. E., Withers, B., McGuire, H. M., Luciani, F., Singh, M., Hughes, B., Gloss, B.,
Kliman, D., Ma, C. K. K., Panicker, S., Bishop, D., Dubosq, M. C., Li, Z., Avdic, S., Micklethwaite, K., Blyth,
- Prophylactic antigen-specific T-cells targeting seven viral and fungal pathogens after allogeneic
haemopoietic stem cell transplant. Clinical and Translational Immunology (2021) 10 3 e1249
Singh, M., Jackson, K. J. L., Wang, J. J., Schofield, P., Field, M. A., Koppstein, D., Peters, T. J., Burnett, D.
L., Rizzetto, S., Nevoltris, D., Masle-Farquhar, E., Faulks, M. L., Russell, A., Gokal, D., Hanioka, A.,
Horikawa, K., Colella, A. D., Chataway, T. K., Blackburn, J., Mercer, T. R., Langley, D. B., Goodall, D. M.,
Jefferis, R., Gangadharan Komala, M., Kelleher, A. D., Suan, D., Rischmueller, M., Christ, D., Brink, R.,
Luciani, F., Gordon, T. P., Goodnow, C. C., Reed, J. H. Lymphoma Driver Mutations in the Pathogenic
Evolution of an Iconic Human Autoantibody Cell (2020) 180 5 878-894 e19
Castellano-Gonzalez, G., McGuire, H. M., Luciani, F., Clancy, L. E., Li, Z., Avdic, S., Hughes, B., Singh,
M., Fazekas de St Groth, B., Renga, G., Pariano, M., Bellet, M. M., Romani, L., Gottlieb, D. J. Rapidly
expanded partially HLA DRB1-matched fungus-specific T cells mediate in vitro and in vivo antifungal
activity Blood Adv (2020) 4 14 3443-3456
Lee, K. H., Gowrishankar, K., Street, J., McGuire, H. M., Luciani, F., Hughes, B., Singh, M., Clancy, L. E.,
Gottlieb, D. J., Micklethwaite, K. P., Blyth, E. Ex vivo enrichment of PRAME antigen-specific T cells for
adoptive immunotherapy using CD137 activation marker selection Clinical & Translational Immunology
(2020) 9 10
Singh, M., Al-Eryani, G., Carswell, S., Ferguson, J.M., Blackburn, J., Barton, K., Roden, D., Luciani,
F., Phan, Giang T., Junankar, S., Jackson, K., Goodnow, C.C., Smith, M.A., Swarbrick, A. Highthroughput
targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of
lymphocytes Nat Commun (2019) 10 1 3120
Rizzetto, S., Koppstein, D.N.P., Samir, J., Singh, M., Reed, J.H., Cai, C.H., Lloyd, A.R., Eltahla, A.A.,
Goodnow, C.C., Luciani, F. B-cell receptor reconstruction from single-cell RNA-seq with VDJPuzzle
Bioinformatics (2018) 34 16 2846-2847
Wirasinha, R.C., Singh, M., Archer, S.K., Chan, A., Harrison, P.F., Goodnow, C.C., Daley, S.R.
alphabeta T-cell receptors with a central CDR3 cysteine are enriched in CD8alphaalpha intraepithelial
lymphocytes and their thymic precursors Immunol Cell Biol (2018) 96 6 553-561
Fisher, C.R., Sutton, H.J., Kaczmarski, J.A., McNamara, H.A., Clifton, B., Mitchell, J., Cai, Y., Dups,
J.N., D'Arcy, N.J., Singh, M., Chuah, A., Peat, T.S., Jackson, C.J., Cockburn, I.A. T-dependent B cell
responses to Plasmodium induce antibodies that form a high-avidity multivalent complex with the
circumsporozoite protein PLoS Pathog (2017) 13 7 e1006469
Andrews, T.D., Whittle, B., Field, M.A., Balakishnan, B., Zhang, Y., Shao, Y., Cho, V., Kirk, M., Singh,
M., Xia, Y., Hager, J., Winslade, S., Sjollema, G., Beutler, B., Enders, A., Goodnow, C.C. Massively
parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate
source for thousands of new mouse models Open Biol (2012) 2 5 120061