Dr David Croucher

Group Leader - Network Biology

Dr David Croucher

David completed his undergraduate studies and PhD research at the University of Wollongong, followed by a post-doctoral position at the Garvan Institute, all of which focused on the role that individual proteins play in the behaviour of cancer cells. Pursuing the idea that these cancer-related prote

Biography

David completed his undergraduate studies and PhD research at the University of Wollongong, followed by a post-doctoral position at the Garvan Institute, all of which focused on the role that individual proteins play in the behaviour of cancer cells. Pursuing the idea that these cancer-related proteins do not act in isolation, but are actually embedded within dynamic networks, David undertook a second post-doctoral position at Systems Biology Ireland in University College Dublin.

After establishing an independent research group at Systems Biology Ireland, David received a Future Research Leader grant from Cancer Institute NSW and has now returned to the Garvan Institute as head of the Network Biology group. The focus of his research group is the investigation of network level aberrations induced by oncogenes and oncogenic mutations, ultimately aiming to elucidate how altered network behaviour leads to therapeutic resistance. 

David completed his undergraduate studies and PhD research at the University of Wollongong, followed by a post-doctoral position at the Garvan Institute, all of which focused on the role that individual proteins play in the behaviour of cancer cells. Pursuing the idea that these cancer-related proteins do not act in isolation, but are actually embedded within dynamic networks, David undertook a second post-doctoral position at Systems Biology Ireland in University College Dublin.

After establishing an independent research group at Systems Biology Ireland, David received a Future Research Leader grant from Cancer Institute NSW and has now returned to the Garvan Institute as head of the Network Biology group. The focus of his research group is the investigation of network level aberrations induced by oncogenes and oncogenic mutations, ultimately aiming to elucidate how altered network behaviour leads to therapeutic resistance. 

Awards and Honours

2017 - Collaboration Achievement Award - Irish Laboratory Awards
2015 - Young Garvan Award
2015 - Pilot and Seed Funding, Sydney Catalyst
2015 - Research Equipment Grant, Cancer Institute NSW
2014 - Future Research Leader Fellowship, Cancer Institute NSW
2013 - Young Leader of the Year (Finalist), Irish Laboratory Awards
2013 - Pilot Grant, Breast Cancer Campaign UK
2012 - Starting Investigator Research Grant, Science Foundation Ireland and Marie Curie Actions COFUND
2007 - Cancer Institute Early Career Development Fellowship, Cancer Institute NSW
2006 - Premiers Award for Outstanding Cancer Research Scholar, Cancer Institute NSW
2005 - Cancer Institute Research Scholar Award, Cancer Institute NSW
2004 - International Society for Fibrinolysis and Proteolysis Special Achievement Award - 17th International Congress on Fibrinolysis and Proteolysis, Melbourne, Victoria

Education

2007 - PhD - University of Wollongong, Australia
2003 - Bachelor of Biotechnology (1st Class Honours) - University of Wollongong, Australia

Selected Publications

Kennedy SP*, Han JZR*, Portmann N, Nobis M, Hastings JF, Murphy KJ, Latham SL, Cadell A, Miladinovic D, Marriott G, O-Donnell Y, Shearer RF, Williams J, Garcia Munoz A, Cox TR, Watkins DN, Saunders DN, Timpson P, Lim E, Kolch W, Croucher DR. (2019) Targeting promiscuous heterodimerization overcomes innate resistance to ERBB2 dimerization inhibitors in breast cancer. Breast Cancer Research, 21(43).

Hastings JF, Skinhas JN, Fey D, Croucher DR*, Cox TR*. (2019) The extracellular matrix as a key regulator of intracellular signalling networks. British Journal of Pharmacology (Invited Review).  In Press.

Hastings JF, Han JZR, Shearer RF, Kennedy SP, Iconomou M, Saunders DN, Croucher DR. (2018) Dissecting multi-protein signaling complexes by Bimolecular Complementation Affinity Purification (BiCAP). J. Vis. Exp. (136), e57109, doi:10.3791/57109

Marini KD, Croucher DR, McCloy R, Gonzalez-Rajal A, Hastings JF, Jayasekara S, Alamgeer M, Boolell V, Vaghijiani V, Szczepny A, Han JZR, Waugh T, Lee HC, Oakes SR, Beena K, Harrison C, Lorensuhewa N, Kita B, Barrow R, Robinson BW, deKretser DM, Wu J, Ganju V, Burgess AJ, Martelotto LG, Rossello FJ, Cain JE, Watkins DN. Inhibition of Activin Signaling Increases the Therapeutic Index of Platinum Chemotherapy in Lung Adenocarcinoma. Science Translational Medicine, 10(451):eaat3504

Conway JRW, Warren SC, Herrmann D, Shearer RF, Killen M, Melenec P, Pinese M, Magenau A, Haigh JJ, Wang Y, Croucher DR, Sansom OJ, Pajic M, Morton JP, Timpson P. (2018) Intravital imaging to monitor therapeutic response in moving hypoxic regions resistant to PI3K pathway targeted therapies in pancreatic cancer. Cell Reports, 23(11): 3312-3326.

Kennedy SP, Hastings JF, Han JZR, Croucher DR. (2016) The under-appreciated promiscuity of the epidermal growth factor receptor family. Frontiers in Cell and Developmental Biology, 4:88.

Croucher DR#, Iconomou M, Hastings JF, Kennedy SP, Han JZR, Shearer R, McKenna J, Wan A, Lau J, Aparicio S, Saunders DN#.  (2016) Bimolecular complementation affinity purification (BiCAP) reveals dimer-specific protein interactions for ERBB2 dimers. Science Signaling, 9(436): ra69.

Rogers S, Fey D, McCloy RA, Parker BL, Mitchell NJ, Payne RJ, Daly RJ, James DE, Watkins DN, Croucher DR, Burgess A. (2016) PP1 initiates the dephosphorylation of MASTL, triggering mitotic exit and bistability in human cells. Journal of Cell Science, 129(7):1340-1354.

Erami Z*, Herrmann D*, Warren SC, Nobis M, McGhee EJ, Lucas M, Leung W, Reischmann N, Mrowinska A, Schwarz J, Kadir S, Conway JRW, Vennin C, Karim SA,  Campbell AD, Gallego-Ortega D, Magenau A, Murphy KJ, Ridgway RA, Law AM, Walters SN, Grey ST, Croucher DR, Zhang L, Herzog H, Hardeman EC, Gunning PW, Ormandy CJ, Jeffry Evans TR, Strathdee D,  Sansom , Morton JP, Anderson KI*, Timpson*. (2016) Intravital FRAP imaging using an E-cadherin-GFP mouse reveals disease and drug-dependent dynamic regulation of cell-cell junctions in live tissue. Cell Reports, 14(1), 152-167.

Fey D, Halasz M, Dreidax D, Kennedy SP, Hastings JF, Rauch N, Garcia Munoz A, Pilkington R, Fischer M, Westermann F, Kolch W, Kholodenko BN, Croucher DR. (2015) Signaling pathway models as biomarkers: Patient-specific simulations of JNK activity predict the survival of neuroblastoma patients. Science Signaling, 8(408), ra130.

Zheng Y, Zhang C, Croucher DR, Soliman MA, Bagshaw R, Pasculescu A, St-Denis N, Taylor L, Tate SA, Hardy RW, Colwill K, Dennis JW, Dai AY, Gingras A, Daly RJ, Pawson T (2013). Temporal regulation of EGF signaling networks by the scaffold protein Shc1. Nature, 499(7457): 166-171.

Croucher DR, Hochgräfe F, Zhang L, Liu L, Lyons RJ, Rickwood D, Tactacan CM, Browne BC, Ali N, Chan H, Shearer RF, Gallego-Ortega D, Saunders DN, Swarbrick A, Daly RJ (2013). Involvement of Lyn and the Atypical Kinase SgK269/PEAK1 in a Basal Breast Cancer Signaling Pathway. Cancer Research, 73(6): 1969-1980.

Fey D, Croucher DR, Kolch W, Kholodenko BN. (2012) Crosstalk and signalling switches in mitogen-activated protein kinase cascades. Frontiers in Physiology, 3:355.

Hochgräfe F, Zhang L, O’Toole S, Browne BC, Pinese M, Porta Cubas A, Lehrbach GM, Croucher DR, Rickwood D, Boulghourjian A, Shearer R, Nair R, Swarbrick A, Faratian D, Mullen P, Harrison DJ, Biankin AV, Sutherland RL, Raftery MJ, Daly RJ (2010). Tyrosine phosphorylation profiling reveals the signaling network characteristics of basal breast cancer cells. Cancer Research, 70(22): 9391-401.

Croucher DR, Rickwood D, Tactacan CM, Musgrove EA, Daly RJ (2010). Cortactin modulates RhoA activation and expression of Cip/Kip cyclin dependent kinase inhibitors to promote cell cycle progression in 11q13-amplified head and neck squamous cell carcinoma cells. Molecular and Cellular Biology, 30(21): 5057-70.

Cao XR, Lill NL, Boase N, Shi PP, Croucher DR, Shan H, Qu J, Sweezer EM, Place T, Kirby PA, Daly RJ, Kumar S, Yang B. (2008) Nedd4 controls animal growth by regulating IGF-I signaling. Science Signaling. 1(38):ra5.

Croucher DR, Saunders DN, Lobov S, Ranson M. (2008) Revisiting the Biological Roles of PAI-2 (SerpinB2) in Cancer.  Nature Reviews Cancer. 8(7):535-45.

Croucher DR, Saunders DN, Stillfried GE, Ranson M. (2007) A Structural Basis for Differential Cell Signaling by PAI-1 and PAI-2 in Breast Cancer cells. Biochemical Journal. 408(2):203-10.

Croucher D, Saunders DN, Ranson M. (2006) The PAI-2:uPA Complex: A New Ligand for the Lipoprotein Receptor-Related Protein (LRP). Journal of Biological Chemistry. 281(15):10206-10213.